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Similar articles. Instead, it is an attempt to look into the present and towards the future of how different combinations of electron microscopy methods that visualize cryogenically preserved biological samples with different levels of complexity can bring us closer to our ultimate dream of understanding, mechanistically, how macromolecules operate inside our cells to generate the adaptive and responsive complexity of the biological world. The discussion on whether and how to make available tomographic data is still open. More recently, methods have emerged combining microfluidic single-cell extraction with single-particle analysis by EM to characterize protein complexes from individual Caenorhabditis elegans embryos In vitro reconstitution of the PIC-mediator supracomplex on chromatinized core promoter DNA from individual complexes left allowed cryo-EM analysis that led to a structural model for the full functional assembly right